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The subdigital adhesive pads of Caribbean Anolis lizards are considered to be a key innovation that permits occupation of novel ecological niches. Although previous work has demonstrated that subdigital pad morphology and performance vary with habitat use, such investigations have only considered the macroscale aspects of these structures (e.g. pad area). The morphological agents of attachment, however, are arrays of hair-like fibres (setae) that terminate in an expanded tip (spatula) and have not been examined in a similar manner. Here we examine the setal morphology and setal field configuration of ecologically distinct species of the monophyletic Jamaican Anolis radiation from a functional and ecological perspective. We find that anoles occupying the highest perches possess greater setal densities and smaller spatulae than those exploiting lower perches. This finding is consistent with the concept of contact splitting, whereby subdivision of an adhesive area into smaller and more densely packed fibres results in an increase in adhesive performance. Micromorphological evidence also suggests that the biomechanics of adhesive locomotion may vary between Anolis ecomorphs. Our findings indicate that, in a similar fashion to macroscale features of the subdigital pad, its microstructure may vary in relation to performance and habitat use in Caribbean Anolis.

 

An operando characterization of electrode materials under electrochemical reaction conditions is important for their further development. X-ray absorption spectroscopy (XAS) presents a unique opportunity in this regard as the absence of a vacuum chamber in this technique makes it possible to collect spectroscopy data using user-designed operando cells. In the current study, the design and performance of an operando XAS cell are evaluated for characterizing solid oxide electrolysis cell working electrodes under a reaction environment that mimics high-temperature ammonia production conditions from H 2 O and N 2 . Sr 2 FeMoO 6−x N x (SFMON)-type double perovskite oxides were used as the cathode materials in these experiments. The operando cell contained a sample stage with a turnable head so that XAS data can be collected at different angles between the electrode and the X-ray beam with an accuracy of 0.5°. The mechanism to adjust the angle of incidence of the beam on the sample allows control over the depth of penetration of the X-ray photons into the electrode. At low angles, it becomes possible to collect surface sensitive data, which is of great importance as the electrochemical processes are believed to take place on the surface of the electrodes. Sr K-edge and Fe K-edge XAS collected at 2° and 45° angles showed that these the oxidation state changes occurring in these elements are different in the near-surface region compared to the bulk of the electrode. Such an ability to distinguish between the surface and bulk properties of the electrode during real reaction environment will help to understand the underlying phenomena better, which will enable electrode design targeted towards the reactions of interest. 

Over the next 5 yr, the Dark Energy Spectroscopic Instrument (DESI) will use 10 spectrographs with 5000 fibers on the 4 m Mayall Telescope at Kitt Peak National Observatory to conduct the first Stage IV dark energy galaxy survey. Atz< 0.6, the DESI Bright Galaxy Survey (BGS) will produce the most detailed map of the universe during the dark-energy-dominated epoch with redshifts of >10 million galaxies spanning 14,000 deg². In this work, we present and validate the final BGS target selection and survey design. From the Legacy Surveys, BGS will target anr< 19.5 mag limited sample (BGS Bright), a fainter 19.5 <r< 20.175 color-selected sample (BGS Faint), and a smaller low-zquasar sample. BGS will observe these targets using exposure times scaled to achieve homogeneous completeness and cover the footprint three times. We use observations from the Survey Validation programs conducted prior to the main survey along with simulations to show that BGS can complete its strategy and make optimal use of “bright” time. BGS targets have stellar contamination <1%, and their densities do not depend strongly on imaging properties. BGS Bright will achieve >80% fiber assignment efficiency. Finally, BGS Bright and BGS Faint will achieve >95% redshift success over any observing condition. BGS meets the requirements for an extensive range of scientific applications. BGS will yield the most precise baryon acoustic oscillation and redshift-space distortion measurements atz< 0.4. It presents opportunities for new methods that require highly complete and dense samples (e.g.,N-point statistics, multitracers). BGS further provides a powerful tool to study galaxy populations and the relations between galaxies and dark matter.

 

Sero-surveillance can monitor and project disease burden and risk. However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool. False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries. Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity. Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity. Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays. ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein. The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro. Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity. Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.

 

In 2021 May, the Dark Energy Spectroscopic Instrument (DESI) began a 5 yr survey of approximately 50 million total extragalactic and Galactic targets. The primary DESI dark-time targets are emission line galaxies, luminous red galaxies, and quasars. In bright time, DESI will focus on two surveys known as the Bright Galaxy Survey and the Milky Way Survey. DESI also observes a selection of “secondary” targets for bespoke science goals. This paper gives an overview of the publicly available pipeline (desitarget) used to process targets for DESI observations. Highlights include details of the different DESI survey targeting phases, the targeting ID (TARGETID) used to define unique targets, the bitmasks used to indicate a particular type of target, the data model and structure of DESI targeting files, and examples of how to access and use thedesitargetcode base. This paper will also describe “supporting” DESI target classes, such as standard stars, sky locations, and random catalogs that mimic the angular selection function of DESI targets. The DESI target-selection pipeline is complex and sizable; this paper attempts to summarize the most salient information required to understand and work with DESI targeting data.

 

Experimental archaeology continues to mature methodologically and theoretically. Around the world, practitioners are increasingly using modern materials that would have been unavailable to prehistoric people in archaeological experiments. The use of a modern material substitute can offer several benefits to experimental method, design, control, replicability, feasibility, and cost, but it should be directly compared to its “traditional” analogue to understand similarities and differences. Here, aluminum is introduced as a substitute for chert in prehistoric ballistics research because, critically, aluminum is safe, inexpensive, easy to process, and it and chert possess densities that differ by less than 4%. The aluminum casting process for replicating stone artifacts is presented, and it is shown that the aluminum castings are essentially identical in form, flake-scar patterning, and mass to their stone counterparts. We then present a proof-of-concept ballistics experiment that demonstrates no difference between aluminum and stone points in terms of target penetration.